β-Gal Staining Kit

β-Gal Staining Kit

The β-Gal Staining Kit allows you to determine the percentage of transfected cells expressing lacZ. lacZ is a bacterial gene often used as a reporter construct in eukaryotic transfection experiments. The gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates and its activity is easily assayed. β-galactosidase catalyzes the hydrolysis of X-gal, producing a blue color that can be easily visualized under a microscope.

Contents

• 10X PBS (Phosphate Buffered Saline): 60 mL
• X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside): 150 mg
• Staining Solution A: 1.5 mL
• Staining Solution B: 1.5 mL
• Staining Solution C: 1.5 mL
• 10X Fixative Solution: 15 mL
• pcDNA3.1/His/LacZ Control Vector: 10 µg