β-Gal Staining Kit
On demand
Order number :
K146501
β-Gal Staining Kit
The β-Gal Staining Kit allows you to determine the percentage of transfected cells expressing lacZ. lacZ is a bacterial gene often used as a reporter construct in eukaryotic transfection experiments. The gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates and its activity is easily assayed. β-galactosidase catalyzes the hydrolysis of X-gal, producing a blue color that can be easily visualized under a microscope.
Contents
- 10X PBS (Phosphate Buffered Saline): 60 mL
- X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside): 150 mg
- Staining Solution A: 1.5 mL
- Staining Solution B: 1.5 mL
- Staining Solution C: 1.5 mL
- 10X Fixative Solution: 15 mL
- pcDNA3.1/His/LacZ Control Vector: 10 µg
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