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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli, 10 Reactions

Out of stock
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 10 Reactions
Order number : K4575J10
($) On demand

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli, 10 Reactions


The TOPO® TA Cloning® Kits for Sequencing provide a highly efficient, 5-minute, one-step cloning strategy ('TOPO® Cloning') for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4-TOPO® TA vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction, and are available with a variety of competent cells, or no competent cells, depending on your needs and budget. These kits include everything necessary to clone and select recombinant vectors containing your PCR fragment of choice

Features

  • Get more sequence—allows for more insert sequence and less vector seuquence when using standard sequencing primers
  • Fast and easy—go from PCR to clones in just 3 steps and in as little as 5 minutes hands-on time
  • Efficient—obtain up to 95% clones with correct insert
  • Proven—reliable performance for over a decade with over 4,000 citations

 

Contents

  • pCR™4-TOPO™ or pCR™4Blunt-TOPO™ Vector
  • Salt Solution
  • dNTPs
  • Control Template and Primers
  • T3, T7, M13 (-20) Forward and M13 Reverse Primers
  • One Shot™ Chemically Competent or Electrocomp™ E. coli
  • S.O.C. Medium
  • pUC19 Control Plasmid
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