Invitrogen™ β-Gal Assay Kit

Invitrogen™ β-Gal Assay Kit

The β-Gal Assay Kit provides the reagents required to quickly measure the levels of active β-galactosidase expressed in cells transfected with plasmids expressing the lacZ gene. lacZ is a bacterial gene often used as a reporter construct in eukaryotic transfection experiments. The gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates and its activity is easily assayed. β-galactosidase catalyzes the hydrolysis of β-galactosidase such as ortho-nitrophenyl-D-galactopyranoside (ONPG). Hydrolysis of ONPG to the ONP anion produces a bright yellow color with a peak absorbance at 420 nm that can be quantified using a spectrophotometer.

10.7 gene expression

Contents

• 10X PBS (Phosphate Buffered Saline): 60 mL
• 1X Lysis Buffer: 10 mL
• 10X Cleavage Buffer: 25 mL
• β-mercaptoethanol: 750 µL
• ONPG: 2 x 5 mL
• Stop Buffer: 55 mL