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Invitrogen™ pAd/CMV/V5-DEST™ Gateway™ Vector Kit

On demand
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 6 µg
Order number : V49320
(Ex-work price)
$ On demand

Invitrogen™ pAd/CMV/V5-DEST™ Gateway™ Vector Kit

 

The pAd⁄CMV⁄V5-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ adenoviral expression vector, pAd⁄CMV⁄V5-DEST™ vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where constitutive, high-level expression is driven by the CMV promoter.

 

Advantages

  • High efficiency and rapid recombination cloning
  • Produces high titer adenoviral stocks
  • Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo
  • Produces replication-incompetent virus for enhanced biosafety of the system
  • Amenable for use in high-throughput applications

 

Features

  • Gateway® Technology for efficient and rapid cloning
  • CMV promoter for high-level constitutive expression of gene of interest
  • Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions
  • Herpes Simplex Virus thymidine kinase (TK) polyadenylation sequence for efficient transcription termination and polyadenylation of mRNA
  • V5 epitope for detection of recombinant protein
  • Ampicillin selection marker

 

Contents

  • pAd⁄CMV⁄V5-DEST™ Gateway® Vector: 40 µL
  • pAd⁄CMV⁄V5-GW⁄lacZ control plasmid: 10 µL
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