Invitrogen™ pAd/CMV/V5-DEST™ Gateway™ Vector Kit
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
6 µg
Order number :
V49320
Invitrogen™ pAd/CMV/V5-DEST™ Gateway™ Vector Kit
The pAd⁄CMV⁄V5-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ adenoviral expression vector, pAd⁄CMV⁄V5-DEST™ vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where constitutive, high-level expression is driven by the CMV promoter.
Advantages
- High efficiency and rapid recombination cloning
- Produces high titer adenoviral stocks
- Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo
- Produces replication-incompetent virus for enhanced biosafety of the system
- Amenable for use in high-throughput applications
Features
- Gateway® Technology for efficient and rapid cloning
- CMV promoter for high-level constitutive expression of gene of interest
- Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions
- Herpes Simplex Virus thymidine kinase (TK) polyadenylation sequence for efficient transcription termination and polyadenylation of mRNA
- V5 epitope for detection of recombinant protein
- Ampicillin selection marker
Contents
- pAd⁄CMV⁄V5-DEST™ Gateway® Vector: 40 µL
- pAd⁄CMV⁄V5-GW⁄lacZ control plasmid: 10 µL
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