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Gibco™ Bac-to-Bac™ Vector Kit

On demand
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 1 Kit
Order number : 10360014
(Ex-work price)
$ On demand

Gibco™ Bac-to-Bac™ Vector Kit

 

The Bac-to-Bac® Vector Kit contains a pFastBac™ 1 vector, as well an expression control vector, intended for use as part of the Bac-to-Bac® Baculovirus Expression System (Cat. No. 10359-016), which enables the efficent production of recombinant baculovirus for expression testing in insect cells. The Bac-to-Bac® System relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells

Features

  • Time-saving expression bacmid. With Bac-to-Bac®, the expression cassette of the pFastBac™ vector recombines with the parent bacmid in DH10Bac™ E. coli
  • Competent Cells (not included with this vector kit) to form an expression bacmid. The bacmid is then transfected into insect cells for production of recombinant
  • baculovirus particles.
  • Easy colony screening. The parent bacmid in DH10Bac™ E. colicontains a segment of the lacZα gene. The lacZα gene is disrupted upon transposition of the
  • expression cassette into the bacmid allowing for blue/white selection of recombinants. This makes identification of recombinant colonies easy.
  • The Bac-to-Bac® Baculovirus Expression System is designed for fast, small-scale production of recombinant baculovirus. The pFastBac™ 1 vector provided by this
  • kit offers the strong polyhedrin promoter for protein expression and a large multiple cloning site for simplified cloning.

Contents

  • pFastBac™1: 20 μL
  • pFastBac™-Gus: 20 μL
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