Gibco™ Bac-to-Bac™ Vector Kit
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
1 Kit
Order number :
10360014
Gibco™ Bac-to-Bac™ Vector Kit
The Bac-to-Bac® Vector Kit contains a pFastBac™ 1 vector, as well an expression control vector, intended for use as part of the Bac-to-Bac® Baculovirus Expression System (Cat. No. 10359-016), which enables the efficent production of recombinant baculovirus for expression testing in insect cells. The Bac-to-Bac® System relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells
Features
- Time-saving expression bacmid. With Bac-to-Bac®, the expression cassette of the pFastBac™ vector recombines with the parent bacmid in DH10Bac™ E. coli
- Competent Cells (not included with this vector kit) to form an expression bacmid. The bacmid is then transfected into insect cells for production of recombinant
- baculovirus particles.
- Easy colony screening. The parent bacmid in DH10Bac™ E. colicontains a segment of the lacZα gene. The lacZα gene is disrupted upon transposition of the
- expression cassette into the bacmid allowing for blue/white selection of recombinants. This makes identification of recombinant colonies easy.
- The Bac-to-Bac® Baculovirus Expression System is designed for fast, small-scale production of recombinant baculovirus. The pFastBac™ 1 vector provided by this
- kit offers the strong polyhedrin promoter for protein expression and a large multiple cloning site for simplified cloning.
Contents
- pFastBac™1: 20 μL
- pFastBac™-Gus: 20 μL
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