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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells, 10 Reactions

Out of stock
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 10 Reactions
Order number : 450071
($) On demand

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells, 10 Reactions


TOPO® TA Cloning® kits for sequencing provide a highly efficient, 5-minute cloning reaction for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4-TOPO® TA vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction, and are available with a variety of competent cells, or no competent cells, depending on your needs and budget. These kits include the tools necessary to clone and select recombinant vectors containing your PCR fragment of choice.


Features

  • Get more sequence—allows for more insert sequence and less vector sequence when using standard sequencing primers
  • Fast and easy—go from PCR to clones in just 3 steps, including a 5-minute cloning reaction
  • Efficient—obtain up to 95% clones with correct insert
  • Proven—enabling reliable performance for over a decade with over 20,000 citations

 

Contents

  • Topoisomerase I-activated pCR™4-TOPO™ vector
  • PCR buffer
  • Salt solution
  • dNTPs
  • Control template
  • T3, T7, M13 forward, and M13 reverse primers
  • Control PCR primers
  • Sterile water
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