Invitrogen™ pcDNA™3.1/Hygro(-) Mammalian Expression Vector
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
20 µg
Order number :
V87520
Invitrogen™ pcDNA™3.1/Hygro(-) Mammalian Expression Vector
This pcDNA™3.1/Hygro(-) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Hygromycin selectable marker and a reverse-orientation multiple cloning site.
The pcDNA™3.1 Expression Vector Family
Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections.
Features
- Cytomegalovirus (CMV) enhancer-promoter for high-level expression
- Large multiple cloning site in either forward (+) or reverse (-) orientations
- Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
- SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e., COS-1 and COS-7)
- Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
Contents
- pcDNA™3.1/Hygro (–) 20 μg
- pDNA™3.1/Hygro/lacZ control 20 μg
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