Invitrogen™ Amplex™ Red Glutamic Acid/Glutamate Oxidase Assay Kit

Invitrogen™ Amplex™ Red Glutamic Acid/Glutamate Oxidase Assay Kit

The Amplex™ Red Glutamic Acid/Glutamate Oxidase Assay Kit provides a sensitive and simple method for detecting glutamic acid/glutamate oxidase activity using a fluorescence microplate reader or fluorometer.

The Amplex™ Red Glutamic Acid/Glutamate Oxidase Assay Kit provides an ultrasensitive method for detecting glutamic acid or for continuously monitoring glutamate oxidase activity using a fluorescence microplate reader or fluorometer. In the assay, L-glutamic acid is oxidized by glutamate oxidase to produce α-ketoglutarate, NH₃, and hydrogen peroxide. L-alanine and L-glutamate–pyruvate transaminase are included in the reaction to regenerate L-glutamic acid by transamination of α-ketoglutarate, resulting in multiple cycles of the initial reaction and a significant amplification of the hydrogen peroxide produced. The hydrogen peroxide reacts with 10-acetyl-3,7-dihydroxyphenoxazine (Amplex™ Red reagent) in a 1:1 stoichiometric ratio in a reaction catalyzed by horseradish peroxidase (HRP) to generate the highly fluorescent product resorufin.

Features

• Detect concentrations as low as 10 nM L-glutamic acid or 40 µU/mL of purified L-glutamate oxidase
• Format allows for multiple time point measurements
• Designed for minimal autofluorescence interference