Invitrogen™ Anza™ T4 DNA Ligase Master Mix, 200 Reactions

Invitrogen™ Anza™ T4 DNA Ligase Master Mix, 200 Reactions

The Invitrogen Anza™ T4 DNA Ligase Master Mix facilitates the joining of abutted 5’-phosphate and 3’-hydroxyl termini in duplex DNA through the formation of a phosphodiester bond. It can be used to join DNA fragments with both sticky ends and blunt ends, and to repair nicks in double-stranded DNA with 3'-hydroxyl and 5'-phosphate ends. Anza T4 DNA Ligase is formulated as a 4X concentrated master mix. Ligation can be performed with DNA in water, TE, elution buffer, or 1X Anza™ buffers.

Features

• Ligation complete in 15 minutes at room temperature
• A single ligase master mix for both sticky-end and blunt-end ligation reactions
• Ready-to-use master mix format reduces pipetting steps
• 4X concentration enables more dilute DNA to be used in ligation reaction without concentrating

Contents

• 1 mL Anza T4 DNA Ligase Master Mix