Invitrogen™ pAd/PL-DEST™ Gateway™ Vector Kit

Invitrogen™ pAd/PL-DEST™ Gateway™ Vector Kit

The pAd⁄PL-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ adenoviral expression vector, pAd⁄PL-DEST® vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where expression is driven by a promoter of choice. Alternatively, the vector may also be used to express small RNA molecules from their appropriate promoters.

Advantages

• High efficiency and rapid recombination cloning
• Produces high titer adenoviral stocks
• Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo
• Allows gene of interest to be controlled by a promoter of choice
• Produces replication-incompetent virus for enhanced biosafety of the system
• Amenable for use in high-throughput applications

Features

• Gateway® Technology for efficient and rapid cloning
• Promoterless vector that allows gene of interest to be controlled by a promoter of choice
• Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions
• Ampicillin selection marker

Contents

• pAd⁄PL-DEST™ Vector 150 ng⁄µl in TEBuffer, pH 8.0. 40 µl (Store at -20°C)
• pAd⁄CMV⁄V5-GW⁄lacZ control plasmid 1 µg⁄µl in TE Buffer, pH 8.0. 10 µl