Invitrogen™ Biotin-14-dATP

Invitrogen™ Biotin-14-dATP

Biotin-14-dATP is a patented dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker. The long, 14-atom spacer arm allows accessibility to streptavidin for sensitive detection of probe-target hybrids in in situ and filter-based hybridizations. Biotin-14-dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP, dGTP, and dTTP. Biotin-14-dATP can be added to the 3´ end of DNA using terminal deoxynucleotidyl transferase. The amount supplied is sufficient for labeling up to 50 µg of DNA by nick translation.

Contents

• Biotin-14-dATP is supplied at 0.4 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA