Thermo Scientific™ North2South™ Complete Biotin Random Prime Labeling and Detection Kit

Thermo Scientific™ North2South™ Complete Biotin Random Prime Labeling and Detection Kit

The Thermo Scientific™ North2South™ Biotin Random Prime Labeling Kit provides the components and procedure to successfully prepare biotin-labeled DNA probes for use in Southern blotting and other DNA hybridization methods involving streptavidin detection systems.

This kit includes the North2South Chemiluminescent Detection Kit, which combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results.

Features

• Sensitivity equal to or greater than 32P
• Much faster than DIG detection systems
• Amenable to nearly any DNA or RNA hybridization method
• Optimized hybridization and blocking buffers and protocol steps yield consistent results from experiment to experiment
• High level of light output and consistent low background allows for very short and multiple exposure times using cooled CCD camera systems or film
• Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user
• Specific plaques can be picked out of areas that are very dense with signal due to extremely low background
  
  

Contents

• 1 North2South Chemiluminescent Hybridization and Detection Kit: 17097
• 1 North2South Biotin Random Prime Labeling Kit: 17075