Invitrogen™ CD44 Alexa Fluor™ 488 Conjugate Kit for Live Cell Imaging

Invitrogen™ CD44 Alexa Fluor™ 488 Conjugate Kit for Live Cell Imaging

Molecular Probes® human pluripotent stem cell (PSC) live imaging kits provide reagents for superior imaging of live human PSCs. This CD44 Alexa Fluor® 488 Conjugate Kit includes an optimally labeled primary antibody paired with reduced-background imaging medium (FluoroBrite™ DMEM) for the specific detection of the negative PSC marker CD44. For fixed cell staining of PSC markers and germ layer markers, we recommend the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit and the 3-Germ Layer Immunocytochemistry Kit.

Features

• Detect Expression of a Negative PSC Marker, Powered by the high performance of bright, photostable Alexa Fluor® dyes, this kit enables reliable live-cell detection of CD44. CD44 is absent in PSCs and expressed on the surface of many other cell types, including human and mouse fibroblasts. This antibody conjugate has been demonstrated to show superior staining results when used with FluoroBrite™ DMEM.
• Preserve Cell Health and Maximize Signal, Staining cells in FluoroBrite™ DMEM maximizes cell health and enhances signal-to-noise ratio when performing live-cell fluorescence imaging.
• Stain Live-cell Samples with Confidence, Unlike most other conjugated antibodies, the reagents provided in this kit are 0.2 µm sterile-filtered and tested to be free of common contaminants.
• Combine with Other Markers, To get more information per sample, combine this kit with the TRA-1-60 Alexa Fluor® 555 Conjugate Kit for Live Cell Imaging or the TRA-1-60 Alexa Fluor® 594 Conjugate Kit for Live Cell Imaging.

Content

• 200 μL of Alexa Fluor™ 488 Rat Anti-Human and Mouse CD44 (50x concentration)
• 500 mL FluoroBrite™ DMEM