Invitrogen™ EnzChek™ Ultra Amylase Assay Kit

Invitrogen™ EnzChek™ Ultra Amylase Assay Kit

The EnzChek™ Ultra Amylase Assay Kit provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek™ kit contains a starch derivative—the DQ™ starch substrate—that is labeled with BODIPY™ FL dye to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters.