Thermo Scientific™ High-Select™ Fe-NTA Phosphopeptide Enrichment Kit

Thermo Scientific™ High-Select™ Fe-NTA Phosphopeptide Enrichment Kit

The Thermo Scientific™ High-Select™ Fe-NTA Phosphopeptide Enrichment Kit enables efficient enrichment of phosphorylated peptides from complex and fractionated protein digests for analysis by mass spectrometry (MS).

The High-Select Fe-NTA Phosphopeptide Enrichment Kit enriches phosphopeptides from complex samples using iron-chelate resin in spin columns. Each column can enrich phosphopeptides from 0.5 to 5 mg of total protein digest as starting sample. Together with pre-formulated buffers, the improved and simplified procedure enriches up to 150 µg of phosphopeptides with greater than 90% selectivity in less than 45 minutes.

For use with: Microcentrifuge

Features

• Convenient—pre-formulated buffers for ease-of-use and spin-column format to enable parallel processing of multiple samples
• High binding capacity—each column enriches up to 150 μg of phosphopeptides
• High specificity—recovers phosphopeptides with > 90% selectivity
• Superior recovery—enriches more total and unique phosphopeptides than other commercially available resins
• High coverage—recovers peptides with single and multiple phosphorylation sites

Contents

• Spin columns (30)
• White Luer plug (end cap) (30)
• Binding/Wash Buffer (2 x 20 mL)
• Phosphopeptide Elution Buffer (7 mL)