Invitrogen™ Expressway™ Maxi Cell-Free E. coli Expression System

Invitrogen™ Expressway™ Maxi Cell-Free E. coli Expression System

The Expressway™ Maxi Cell-Free E. coli Expression System uses an efficient, coupled transcription/translation reaction to produce milligram quantities of soluble, functionally active protein in 4-6 hours. The procedure can be performed in a single reaction tube and is easily scalable without the need for specialized equipment. The TOPO TA Cloning® expression vectors (5-min cloning with 95% efficiency) are provided for optimal expression results. In addition to all the advantages of an open expression system, Expressway™ Maxi technology provides a means to produce high levels of recombinant protein that may be easily detected and purified for various downstream applications.

Features

• Milligram level protein production within 4-6 hours
• High-throughput (HTP) compatible
• Optimized vectors
• Simple and fast procedure

Contents

• The Expressway™ Maxi Cell-Free Expression System is designed to perform two hundred 25-µL
• five 1-mL, or one 5-mL (initial volume) reactions or one to several large reactions
• This system includes the E.coli slyD- Extract
• 2.5X IVPS reaction buffer
• feed buffer
• 19 amino acid mix
• methionine
• DNase/RNase-free water
• T7 Enzyme Mix
• a positive expression control vector
• pEXP5-NT/TOPO™ vector
• pEXP5-CT/TOPO™ vector 
• other reagents to facilitate TOPO™ Cloning
• One Shot™ TOP10 Chemically Competent E. coli.