Invitrogen™ Expressway™ Mini Cell-Free Expression System

Invitrogen™ Expressway™ Mini Cell-Free Expression System

The Expressway™ Mini Cell-Free Expression System utilizes an efficient coupled transcription and translation reaction to produce active recombinant protein. This system eliminates the time-consuming steps of cell-based protein production including transformation, cell culture, and expression optimization. In just two hours, you can produce protein suitable for an array of downstream applications and functional studies. The Expressway™ Mini Cell-Free Expression System offers flexibility in experimental design

Features

• Synthesize proteins from both circular plasmid and linear templates (e.g. PCR products)
• Perform high-throughput synthesis of proteins
• Express genes toxic to in vivo systems

Contents

• the IVPS E.coli Extract
• IVPS reaction buffer
• feed buffer
• 19 amino acid mix
• methionine
• DNase/RNase-free water
• RNase A
• T7 Enzyme Mix
• 2-mL reaction tubes
• a positive expression control vector