Invitrogen™ GeneArt™ Gibson Assembly EX Cloning Kit, electrocompetent cells

Invitrogen™ GeneArt™ Gibson Assembly EX Cloning Kit, electrocompetent cells

The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility and success of this approach is suitable for small and large DNA constructs, includes both single and multiple inserts, and has been used to build entire genomes. The resulting products can be used for a variety of downstream applications, including transformation, PCR, and rolling circle amplification (RCA). The GeneArt Gibson Assembly EX Cloning Kit, electrocompetent cells, is a complete kit that includes master mix, positive control, water, and ElectroMAX DH10B electrocompetent E. coli.

Features

• Simple—seamlessly assemble and clone up to 15 DNA fragments in a single reaction
• Efficient—robust efficiency provides successful clones for both simple and challenging constructs
• Flexible—design guidelines allow assembly into any vector of your choice
• Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells
• Trusted—over 4000 citations in the scientific literature highlighting great success

Contents

• Master mix
• Positive control
• Water
• ElectroMAX DH10B electrocompetent E. coli