Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Electrocomp™ E. coli, 50 Reactions
On demand
Order number :
K286040
Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Electrocomp™ E. coli, 50 Reactions
Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR™-Blunt II-TOPO® Vector for 5-minute benchtop ligations without ligase.
Features
- ccdB gene for positive selection (2)
- EcoR I sites flanking the PCR product insertion site for easy excision of inserts
- Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli
- SP6 promoter/primer site for in vitro RNA transcription and sequencing
- M13 forward and reverse primer sites for sequencing or PCR screening
Contents
Box 1:
- Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
- Salt solution
- dNTPs
- Control template
- M13 forward and reverse primers
- Sterile water
Box 2:
- One Shot™ Chemically Competent or Electrocomp™ E. coli
- S.O.C. medium
- Supercoiled pUC19 control plasmid
There are no report
You May Also Like
![](https://laboshop.ae/public/storage/files/9kDO06ptLRNc3Sp3GII0av8DyAJckdJ68SWHzyc6.jpg)
Invitrogen™ GeneArt™ Gibson Assembly EX Cloning Kit, chemically competent cells
(Ex-work price)
$ On demand
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/kUqLZBHb3XwJXF3rcCLEwmYgdOdJQ4ZFGdk9brZ7.jpg)
Invitrogen™ pENTR™/TEV/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli
(Ex-work price)
$ On demand
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/1sOxQnAQ6syEkr91f9Y8zHa1RVdXrNJB3YuFjGlk.png)
Image Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli and PureLink™ Kit
(Ex-work price)
$ On demand
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/63f80b9a-fa24-4d8d-8dd0-420d728abd18.jpg)
Invitrogen™ GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit(with competent cells)
(Ex-work price)
$ 1,182
$ 1,182
On demand
![](https://laboshop.ae/public/storage/files/HfixVorAVkZAlZobDvRnTHiMbb788juC4OrbsQ0w.jpg)
Invitrogen™ GeneArt™ Gibson Assembly HiFi Cloning Kit, electrocompetent cells
(Ex-work price)
$ On demand
$ On demand