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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells, 10 Reactions

Out of stock
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 10 Reactions
Order number : 452640
($) On demand

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells, 10 Reactions


TOPO® TA Cloning® Dual Promoter kits are for fast, efficient cloning and subsequent in vitro transcription. These kits include the pCR™II-TOPO® TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO® cloning is the most reliable method, featuring a 3-step protocol and 5-minute cloning reaction, and yielding up to 95% recombinants.


Features

  • 3´-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 and SP6 promoters for efficient in vitro transcription
  • M13 forward and reverse primer sites for sequencing
  • 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
  • Kanamycin and ampicillin resistance for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants
  • No competent cells included for flexibility and cost-savings

 

Contents

  • Topoisomerase I-activated pCR™II-TOPO™ vector
  • PCR buffer
  • Salt solution
  • dNTPs
  • Control template
  • M13 forward and reverse primers
  • Control PCR primers
  • Sterile water
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