Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells, 10 Reactions
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
10 Reactions
Order number :
452640
Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells, 10 Reactions
TOPO® TA Cloning® Dual Promoter kits are for fast, efficient cloning and subsequent in vitro transcription. These kits include the pCR™II-TOPO® TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO® cloning is the most reliable method, featuring a 3-step protocol and 5-minute cloning reaction, and yielding up to 95% recombinants.
Features
- 3´-T overhangs for direct ligation of Taq-amplified PCR products
- T7 and SP6 promoters for efficient in vitro transcription
- M13 forward and reverse primer sites for sequencing
- 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
- Kanamycin and ampicillin resistance for your choice of selection in E. coli
- Easy blue/white colony screening for selection of recombinants
- No competent cells included for flexibility and cost-savings
Contents
- Topoisomerase I-activated pCR™II-TOPO™ vector
- PCR buffer
- Salt solution
- dNTPs
- Control template
- M13 forward and reverse primers
- Control PCR primers
- Sterile water
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