Invitrogen™ Gateway™ pENTR™ 4 Dual Selection Vector
On demand
Order number :
A10465
Invitrogen™ Gateway™ pENTR™ 4 Dual Selection Vector
Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone.
Fratures
- attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression
- Kozak consensus sequence for efficient translation initiation in eukaryotic systems
- Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR™ 1A Dual Selection, pENTR™3C Dual Selection, and pENTR™11 Dual Selection vectors only)
- rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli
- pUC origin for high-copy replication and maintenance of the plasmid in E. coli
- Kanamycin resistance gene for selection in E. coli
- The ccdB⁄chloramphenicol fusion gene located between the two attL sites for
- o negative selection and
- o Chloramphenicol selection in E. coli
- Kanamycin resistance gene for selection in E. coli
Contents
- 10 µg pENTR™ Dual Selection vector, in 20 ul in TE buffer, pH 8.0.
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