Invitrogen™ pPICZ A, B, & C Pichia Vectors

Invitrogen™ pPICZ A, B, & C Pichia Vectors

The pPICZ A, B, & C Pichia Vectors are designed for simple cloning and selection, high-level expression, and rapid detection and purification of the recombinant protein. These vectors contain the Zeocin™ resistance gene for direct selection of multi-copy integrant strains. By selecting with increasing amounts of Zeocin™, strains with multiple copies of your gene of interest integrated into the genome are obtained. Increasing the number of copies of the gene of interest in a recombinant Pichia strain can result in higher expression levels. The vectors are included in the EasySelect™ Pichia Expression Kit (K1740-01).

Features

• Inducible AOX1 promoter for high-level expression in Pichia pastoris
• c-myc epitope tag for convenient detection with an Anti-myc Antibody
• C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody
• Zeocin™ resistance for direct selection of multi-copy integrants

Contents 

• 20 µg each of pPICZ A, B, & C suspended in 10 mM Tris–HCl, 1 mM EDTA, pH 8.0. A GS115/pPICZ/lacZ Positive Control strain is also included.