Invitrogen™ pBAD/His Kit
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
1 Kit
Order number :
V43001
Invitrogen™ pBAD/His Kit
The pBAD/His Kit provides all of the necessary reagents to express your protein in a tightly regulated fashion. The vector pBAD/His allows you to express your protein with an N-terminal tag.
Three vectors are provided (A, B, and C). Each has the N-terminal tag in a different reading
frame relative to the multiple cloning site to simplify in-frame cloning of your gene.
Features
- The araBAD promoter for tightly regulated expression
- Translation initiation signals optimized for E. coliexpression
- N-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with an Anti-HisG Antibody
- N-terminal Xpress™ epitope for detection and analysis with an Anti-Xpress™ Antibody
- Enterokinase cleavage site for removing the N-terminal tag following purification
Contents
- 20 µg each of pBAD/His A, B, and C vector in TE Buffer, pH 8.0[1] (40 µL at 0.5 µg/µl)
- 20 µg each of pBAD/His/lacZ vector in TE Buffer, pH 8.0 (40 µL at 0.5 µg/µL)
- 1 mL sterile, 20% L-arabinose
- 1 stab LMG194
- 1 stab TOP10