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Invitrogen™ pBAD/His Kit

Out of stock
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 1 Kit
Order number : V43001
($) On demand

Invitrogen™ pBAD/His Kit

 

The pBAD/His Kit provides all of the necessary reagents to express your protein in a tightly regulated fashion. The vector pBAD/His allows you to express your protein with an N-terminal tag. 

Three vectors are provided (A, B, and C). Each has the N-terminal tag in a different reading

frame relative to the multiple cloning site to simplify in-frame cloning of your gene.

 

Features

  • The araBAD promoter for tightly regulated expression
  • Translation initiation signals optimized for E. coliexpression
  • N-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with an Anti-HisG Antibody
  • N-terminal Xpress™ epitope for detection and analysis with an Anti-Xpress™ Antibody
  • Enterokinase cleavage site for removing the N-terminal tag following purification

 

Contents

  • 20 µg each of pBAD/His A, B, and C vector in TE Buffer, pH 8.0[1] (40 µL at 0.5 µg/µl)
  • 20 µg each of pBAD/His/lacZ vector in TE Buffer, pH 8.0 (40 µL at 0.5 µg/µL)
  • 1 mL sterile, 20% L-arabinose
  • 1 stab LMG194
  • 1 stab TOP10
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