Thermo Scientific™ Biotin DecaLabel DNA Labeling Kit, 10 Reactions

Thermo Scientific™ Biotin DecaLabel DNA Labeling Kit, 10 Reactions

Thermo Scientific Biotin DecaLabel DNA Labeling Kit is an advanced system for the efficient synthesis of biotin-labeled DNA probes, based on an improved random-primed labeling method developed by Feinberg and Vogelstein. The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers, ensuring more efficient annealing with DNA at 37°C. Klenow Fragment, exo-, included in the kit, is genetically engineered enzyme with no detectable exonuclease activity. The enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. You can uniformly label any length DNA fragments.
Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or conventional biotin-avidin or biotin-streptavidin detection systems.

Features

• Non-radioactive labeling of DNA
• Efficient priming of labeling reactions with random decamers
• High yields with Klenow Fragment, exo-: no degradation of a labeled probe during reaction
• Includes Label or Dye: Yes
• Labeling Method: Direct Labeling
• Product Type: DNA Labeling Kit
• Detection Method: Biotin-Based
• Final Product Type: DNA
• Format: Kit
• Labeling Target: Genomic DNA, PCR Products, DNA (General)
  

Contents

• Klenow Fragment, exo–, 5 u/μL: 15 μL 
• Decanucleotide in 5X Reaction Buffer: 100 μL 
• Biotin Labeling Mix: 50 μL
• Control Template λ DNA/HindIII fragments,10 ng/μL: 50 μL 
• Biotin-labeled DNA 50 μL of biotin-labeled DNA (λ DNA/HindIII), 5 ng/μl: 50 μL 
• Water, nuclease-free: 1.25 mL