Invitrogen™ MultiShot™ TOP10 Chemically Competent E. coli

Invitrogen™ MultiShot™ TOP10 Chemically Competent E. coli

MultiShot™ TOP10 Chemically Competent E. coli provide transformation efficiencies of >1 x 108 cfu/µg control plasmid DNA, and are suited for high-throughput transformations. MultiShot™ TOP10 Chemically Competent cells are packaged in five 96-well microtiter plates (15 µL aliquots) to simplify high-throughput bacterial transformations. 

Features

• Designed for high-throughput applications—microtiter plate format designed for automated high-throughput cloning
• Compatible with genomic DNA—the mcrA mutation supports efficient transfection of methylated DNA
• Permits rapid screening—the LacZΔM15 allele permits rapid blue/white screening of transfectants on plates containing X-Gal or Bluo-Gal
• Efficient—the endA1 mutation helps reduce endonuclease activity, improving subsequent plasmid DNA yields
• Reliable—the recA1 mutation helps reduce unwanted recombination
• Easy to use for high-throughput transformations

Contents

• MultiShot™ TOP10 Chemically Competent E. coli: Five 96-well plates, 15 µl per well
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 5 bottles, 15 ml each