Invitrogen™ T4 DNA Ligase (1 U/µL), 100 units
On demand
Order number :
15224017
Invitrogen™ T4 DNA Ligase (1 U/µL), 100 units
T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes. Single-stranded nucleic acids are not substrates for this enzyme.
Contents
- T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6)
- 50 mM MgCl2
- 5 mM ATP
- 5 mM DTT
- 25% (w/v) polyethylene glycol-8000]
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