Invitrogen™ pBAD TOPO™ TA Expression Kit, 20 reactions
On demand
Order number :
K430001
Invitrogen™ pBAD TOPO™ TA Expression Kit, 20 reactions
The pBAD TOPO® TA Expression Kit is specifically designed for one-step cloning and regulated prokaryotic expression of Taq-amplified PCR products. Once you've TOPO® Cloned your PCR product, you can go straight to protein expression.
Features
- Linearized, topoisomerase I-activated vector for 5-minute cloning of Taqamplified PCR products
- The araBAD promoter for tightly regulated expression in E. coli V5 epitope tag for detection with an Anti-V5 Antibody
- C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with an Anti-His(C-term) Antibody
- Enterokinase cleavage site for removal of N-terminal leader peptide
Contents
- The pBAD TOPO™ TA box contains all of the reagents necessary for PCR (except Taq polymerase), including 200 ng of topoisomerase I-activated pBAD-TOPO™ vector
- sterile water
- dNTPs
- 10X PCR buffer
- salt solution
- control template and primers
- 20% L-arabinose
- pBAD primers for sequencing or PCR screening
- an expression control plasmid
- The One Shot™ box contains all of the reagents necessary for transformation, including single-use 50 µl aliquots of One Shot™ TOP10 Chemically Competent E. coli
- S.O.C. medium
- a supercoiled control plasmid
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