Invitrogen™ pBAD TOPO™ TA Expression Kit, 20 reactions

Invitrogen™ pBAD TOPO™ TA Expression Kit, 20 reactions

The pBAD TOPO® TA Expression Kit is specifically designed for one-step cloning and regulated prokaryotic expression of Taq-amplified PCR products. Once you've TOPO® Cloned your PCR product, you can go straight to protein expression. 

Features

• Linearized, topoisomerase I-activated vector for 5-minute cloning of Taqamplified PCR products
• The araBAD promoter for tightly regulated expression in E. coli V5 epitope tag for detection with an Anti-V5 Antibody
• C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with an Anti-His(C-term) Antibody
• Enterokinase cleavage site for removal of N-terminal leader peptide

Contents 

• The pBAD TOPO™ TA box contains all of the reagents necessary for PCR (except Taq polymerase), including 200 ng of topoisomerase I-activated pBAD-TOPO™ vector
• sterile water
• dNTPs
• 10X PCR buffer
• salt solution
• control template and primers
• 20% L-arabinose
• pBAD primers for sequencing or PCR screening
• an expression control plasmid
• The One Shot™ box contains all of the reagents necessary for transformation, including single-use 50 µl aliquots of One Shot™ TOP10 Chemically Competent E. coli
• S.O.C. medium
• a supercoiled control plasmid