Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli

Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli

The pENTR™/D-TOPO® Cloning Kits utilize a highly efficient, 5-minute cloning strategy ("TOPO® Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway® System or the MultiSite Gateway® System. Blunt-end PCR products clone directionally at greater than 90% efficiency, with no ligase, post-PCR procedures, or restriction enzymes required.

Features

• Gateway® System Ready - Rapidly shuttle cloned genes between multiple vector systems
• Fast and Easy - Go from PCR to Gateway® Entry clone in just 3 steps and as little as ∼5 minutes hands on time
• Efficient - Achieve over 90% clones with correct insert in the right direction
• Proven - Reliable performance for over a decade

Contents

• Appropriate, ready-to-use TOPO™ vector
• DNTPs
• Salt solution
• Sterile water
• Universal M13 sequencing primers
• TOP10 or Mach1-T1R Chemically E. coli
• S.O.C. Medium
• pUC19 control plasmid