Invitrogen™ pENTR™/SD/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli

Invitrogen™ pENTR™/SD/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli

The pENTR™/SD/D-TOPO® Cloning Kits utilize a highly efficient, 5-minute cloning strategy ("TOPO® Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway® System or the MultiSite Gateway® System. Blunt-end PCR products clone directionally into the pENTR™/SD/D-TOPO® entry vector at greater than 90% efficiency, and include an upstream Shine-Dalgarno sequence for expression in prokaryotes.

Features

• Gateway® System Ready - Rapidly shuttle cloned genes between multiple vector systems
• Fast and Easy - Go from PCR to Gateway® Entry clone in just 3 steps and as little as ∼5 minutes hands on time
• Efficient - Achieve over 90% clones with correct insert in the right direction
• Proven - Reliable performance for over a decade

Contents

• pENTR⁄SD⁄D-TOPO™ vector
• dNTPs
• Salt solution
• Sterile water
• Universal M13 sequencing primers
• OneShot™ TOP10 Chemically E. coli
• S.O.C. Medium
• pUC19 control plasmid