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Invitrogen™ Pichia Expression Kit, original kit

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Order number : K171001
(Ex-work price)
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Invitrogen™ Pichia Expression Kit, original kit


The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastoris. The vectors carry the HIS4 gene for selection of transformants on histidine-deficient medium. The Original Pichia Expression Kit provides all of the tools and reagents needed to express a gene of interest from the AOX1 promoter.


Contents

The Original Pichia Expression Kit includes:

  • pHIL-D2 and pPIC3.5 vectors for intracellular expression: 10 µg 
  • pHIL-S1 and pPIC9 vectors for secreted expression: 10 µg 
  • Pichia pastoris strains GS115 (his4), KM71(arg4 his4 aox1::ARG4)
  • Control strain GS115 Albumin
  • Control strain GS115 β-gal
  • Media (YP and YNB)
  • Spheroplast Transformation Kit
  • 5´ AOX1: 2 µg 
  • 3´ AOX1: 2 µg 
  • α-factor sequencing primers


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