Invitrogen™ pRSET A, B, & C Bacterial Expression Vectors

Invitrogen™ pRSET A, B, & C Bacterial Expression Vectors

The pRSET vector is designed for high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3) E. coli. These cells also produce T7 lysozyme to reduce basal expression of target genes. 

Features

• Bacteriophage T7 promoter for high-level expression
• T7 gene 10 sequence to provide protein stability
• N-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-HisG Antibody
• N-terminal Xpress™ epitope for detection with the Anti-Xpress™ Antibody
• Enterokinase cleavage site for removal of fusion tag

Contents

• 20 μg each of pRSET A, B, and C in TE buffer, pH 8.0* (40 μl each at 0.5 μg/μl) 
• 1 stab TOP10F′
• 1 stab BL21(DE3)pLysS 
• 1 stab BL21(DE3)pLysS containing the pRSET/lacZ control