Invitrogen™ pcDNA™3.2/GW/D-TOPO™ Expression Kit

Invitrogen™ pcDNA™3.2/GW/D-TOPO™ Expression Kit

The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines.

TOPO® Cloning

Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert, especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein.

Features

• Cytomegalovirus (CMV) promoter for high-level expression
• Adapted for Directional TOPO® Cloning, enabling you to use proofreading polymerases and to clone your PCR products in a specific orientation
• Neomycin resistance gene for stable selection
• C-terminal V5 tag for easy detection
• Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Contents

• pcDNA3.2/V5/GW/D-TOPO®: 20 μL
• dNTP Mix: 10 μL
• Salt Solution: 50 μL
• Water: 1 mL
• T7 Promoter Primer: 20 μL
• TK polyA Reverse Primer: 20 μL
• Control PCR Primers: 10 μL
• Control PCR Template: 10 μL
• Control Plasmid pcDNA™3.2/V5/GW-CAT: 10 μL