Invitrogen™ pTracer™-CMV/Bsd Mammalian Expression Vector

Invitrogen™ pTracer™-CMV/Bsd Mammalian Expression Vector

Two Tracer™ mammalian expression vectors are available that express GFP fused to the selectable marker Blasticidin. Blasticidin is a potent selection agent that allows a more rapid generation of stable cell lines than other selection agents. Each vector uses a different set of promoters to express the gene of interest and the Cycle 3 GFP-Blasticidin resistance gene fusion.

The pTracer™-CMV/Bsd vector offers:

• CMV promoter for high-level constitutive expression of your gene of interest
• EF-1 promoter for high-level constitutive expression of the Cycle 3 GFP-Blasticidin resistance fusion

The pTracer™-EF/Bsd vectors offer:

• EF-1 promoter for high-level constitutive expression of your gene of interest
• CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Blasticidin resistance fusion
• C-terminal V5 epitope tag for simple detection of recombinant protein with an Anti-V5 Antibody
• C-terminal 6xHis tag for rapid purification on nickel-chelating resin and simple detection with an Anti-His(C-term) Antibody
• Three reading frames for easier in-frame cloning

Contents 

• 20 µg each of the pTracer™ /Bsd vector
• lacZ expression control are provided supercoiled and lyophilized. pTracer™ /Bsd Kits contain the appropriate pTracer™ vector
• 50 mg of the selection agent Blasticidin