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Invitrogen™ pTracer™-CMV/Bsd Mammalian Expression Vector

Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 20 µg
Order number : V88320
($) On demand

Invitrogen™ pTracer™-CMV/Bsd Mammalian Expression Vector

 

Two Tracer™ mammalian expression vectors are available that express GFP fused to the selectable marker Blasticidin. Blasticidin is a potent selection agent that allows a more rapid generation of stable cell lines than other selection agents. Each vector uses a different set of promoters to express the gene of interest and the Cycle 3 GFP-Blasticidin resistance gene fusion.

The pTracer™-CMV/Bsd vector offers:

  • CMV promoter for high-level constitutive expression of your gene of interest
  • EF-1 promoter for high-level constitutive expression of the Cycle 3 GFP-Blasticidin resistance fusion

The pTracer™-EF/Bsd vectors offer:

  • EF-1 promoter for high-level constitutive expression of your gene of interest
  • CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Blasticidin resistance fusion
  • C-terminal V5 epitope tag for simple detection of recombinant protein with an Anti-V5 Antibody
  • C-terminal 6xHis tag for rapid purification on nickel-chelating resin and simple detection with an Anti-His(C-term) Antibody
  • Three reading frames for easier in-frame cloning

 

Contents 

  • 20 µg each of the pTracer™ /Bsd vector
  • lacZ expression control are provided supercoiled and lyophilized. pTracer™ /Bsd Kits contain the appropriate pTracer™ vector
  • 50 mg of the selection agent Blasticidin
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