Thermo Scientific™ Biotin DecaLabel DNA Labeling Kit, 30 Reactions
On demandThermo Scientific™ Biotin DecaLabel DNA Labeling Kit, 30 Reactions
Thermo Scientific Biotin DecaLabel DNA Labeling Kit is an advanced system for the efficient synthesis of biotin-labeled DNA probes, based on an improved random-primed labeling method developed by Feinberg and Vogelstein. The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers, ensuring more efficient annealing with DNA at 37°C. Klenow Fragment, exo-, included in the kit, is genetically engineered enzyme with no detectable exonuclease activity. The enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. You can uniformly label any length DNA fragments.
Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or conventional biotin-avidin or biotin-streptavidin detection systems.
Features
- Non-radioactive labeling of DNA
- Efficient priming of labeling reactions with random decamers
- High yields with Klenow Fragment, exo-: no degradation of a labeled probe during reaction
- Includes Label or Dye: Yes
- Labeling Method: Direct Labeling
- No. of Reactions: 30
- Product Type: DNA Labeling Kit
- Detection Method: Biotin-Based
- Final Product Type: DNA
- Format: Kit
- Labeling Target: Genomic DNA, PCR Products, DNA (General)
Contents
- Klenow Fragment, exo–, 5 u/μL: 30 μL
- Decanucleotide in 5X Reaction Buffer: 300 μL
- Biotin Labeling Mix: 150 μL
- Control Template λ DNA/HindIII fragments,10 ng/μL: 125 μL
- Biotin-labeled DNA 50 μL of biotin-labeled DNA (λ DNA/HindIII), 5 ng/μl: 125 μL
- Water, nuclease-free: 2x1.25 mL
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