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Invitrogen™ pMIB/V5-His Vector Kit

On demand
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 1 Kit
Order number : V803001
(Ex-work price)
$ On demand

Invitrogen™ pMIB/V5-His Vector Kit

 

pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five™ insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. 

 

Features

  • The OpIE2 promoter for constitutive expression
  • The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein
  • The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
  • C-terminal V5-epitope and polyhistidine tag for detection with Invitrogens Anti-V5 antibody and purification with nickel-chelating resin

 

Contents

  • pMIB/V5-His A, B, and C: 40 μL
  • pMIB/V5-His/CAT: 40 μL
  • OpIE2 Forward Sequencing Primer: 2 μg
  • OpIE2 Reverse Sequencing Primer: 2 μg
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