Invitrogen™ Thiol and Sulfide Quantitation Kit
On demand
Order number :
T6060
Invitrogen™ Thiol and Sulfide Quantitation Kit
The Thiol and Sulfide Quantitation Kit provides an ultrasensitive colorimetric assay for quantitating both protein and nonprotein thiols (also called sulfhydryls or mercaptans). In this assay, thiols or inorganic sulfides reduce a disulfide-inhibited derivative of papain, stoichiometrically releasing the active enzyme. The activity of the enzyme is then measured using the chromogenic papain substrate N-benzoyl-L-argining, p-nitroanilide (L-BAPNA). The enzymatic amplification step in this kit enables researchers to detect as little as 0.2 µM thiol - a sensitivity that is about 100-fold greater than that achieved using Ellman's reagent.
There are no specifications
There are no report
You May Also Like
Invitrogen™ DiFMUP (6,8-Difluoro-4-Methylumbelliferyl Phosphate), 10 x 10 mg
(Ex-work price)
$ 4
$ 4
On demand
Thermo Scientific™ 96-Well Plates for Pierce™ Quantitative Fluorometric Peptide Assay
(Ex-work price)
$ On demand
$ On demand
On demand
Thermo Scientific™ Pierce™ Cypridina Luciferase Flash Assay Kit, 1000 Reactions
(Ex-work price)
$ On demand
$ On demand