Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, with One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells, 20 Reactions
On demand
Order number :
K8050-20
Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, with One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells, 20 Reactions
The TOPO™ XL-2 Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb). The kit uses the linearized and topoisomerase 1-activated pCR™-XL-2-TOPO™ vector, which is compatible with the cloning of blunt-end PCR fragments. Amplification of long PCR fragments is enabled by Platinum™ SuperFi™ Green PCR Master Mix, which is included in the kit. Topoisomerase I activation of the vector allows PCR products to be ligated in just 5 minutes on your bench–top, resulting in high cloning efficiency (up to 90% positive recombinants with 10 kb fragments).
Features
- ccdB gene for positive selection
- EcoR I site flanking the PCR product insertion site for easy excision of inserts
- Ampicillin- and kanamycin-resistance genes for your choice of selection in E. coli
- T7 promoter/priming site for in vitro RNA transcription and sequencing
- T7, T3, and M13 forward and reverse primer sites for sequencing
Contents
Box 1:
- pCR XL-2-TOPO vector
- Salt Solution
- T3 Primer
- T7 Primer
- XL Control PCR Template
- XL Control PCR Primers
- Platinum SuperFi Green PCR Master Mix
- SuperFi GC Enhancer
- Nuclease-free water
Box 2:
- Gel Solubilization Buffer (L3)
- Binding Buffer (B2)
- Wash Buffer (W1)
- Elution Buffer (E1)
- PureLink Clean-up Spin Columns (in Wash Tubes)
- PureLink Recovery/Elution Tubes
Box 3:
- One Shot OmniMAX 2 T1R chemically competent E. coli
- S.O.C. Medium
- pUC 19 Control DNA
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