Invitrogen™ TrueTag™ Donor DNA Kit, 6xHis
On demand
Order number :
A53811
Invitrogen™ TrueTag™ Donor DNA Kit, 6xHis
The Invitrogen TrueTag Donor DNA Kit, 6xHis, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.
Features
Benefits of the TrueTag Donor DNA Kit include:
- One-step PCR amplification to generate donor templates—no cloning required
- Generate transfection-ready DNA donors in hours instead of days
- Enrich up to 100% 6xHis-tagged cells
The TrueTag Donor DNA Kit, 6xHis, provides everything you need to produce a high-quality DNA donor to tag your gene with a selection marker and 6xHis. Simply provide two locus-specific primers to add homology arms, PCR-amplify, and purify for a transfection-ready donor in hours, not days. Each kit is designed to enable you to:
- Tag the carboxy- (C-) or amino- (N-) terminus of your gene of interest
- Choose from puromycin or blasticidin selection markers—whatever best suits your cell model
- Easily remove antibiotic resistance markers with Cre/Lox following cell enrichment
Included in the kit
- Four linear donor templates: N-terminus/puromycin resistance, N-terminus/blasticidin resistance, C-terminus/puromycin resistance, C-terminus/blasticidin resistance
- Phusion Flash High-Fidelity PCR Master Mix
- PCR cleanup columns and buffers
- Positive control primers to tag human beta-actin (ACTB)
Contents
- TrueTag Assembly Kit
- TrueTag Cleanup Kit
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