Invitrogen™ WT Expression Kit, high throughput, 24 Reactions
On demand
Order number :
4440537
Invitrogen™ WT Expression Kit, high throughput, 24 Reactions
The WT Expression Kit is an RNA amplification kit that is designed to generate amplified sense-strand cDNA ready for fragmentation and labeling using the Affymetrix® GeneChip® WT Terminal Labeling Kit.
Features
- Optimized for use with Affymetrix® GeneChip® Human, Mouse, and Rat 1.0 ST Arrays
- Allows RNA samples as small as 50 ng to be analyzed on Affymetrix® GeneChip® Arrays
- Supports a streamlined workflow that does not require a separate rRNA depletion step
- Is ideal for high-sensitivity expression profiling
Contents
- First-Strand Enzyme Mix (-20°C): 50 µl
- First-Strand Buffer Mix (-20°C): 160 µl
- Second-Strand Enzyme Mix (-20°C): 175 µl
- Second-Strand Buffer Mix (-20°C): 400 µl
- IVT Enzyme Mix (-20°C): 210 µl
- IVT Buffer Mix (-20°C): 750 µl
- Control RNA 1 mg/ml (-20°C): 10 µl
- 2nd-Cycle Buffer Mix (-20°C): 425 µl
- Random Primers (-20°C): 100 µl
- 2nd-Cycle Enzyme Mix (-20°C): 275 µl
- RNase H (-20°C): 100 µl
- Nucleic Acid Binding Buffer Concentrate (room temperature): 3.5 ml
- Nucleic Acid Binding Beads (4°C): 700 µl
- Nucleic Acid Wash Solution Concentrate (room temperature): 15 ml
- Elution Solution (4°C or room temperature): 6 ml
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