Invitrogen™ WT Expression Kit, high throughput, 96 Reactions
On demand
Order number :
4440536
Invitrogen™ WT Expression Kit, high throughput, 96 Reactions
The WT Expression Kit is an RNA amplification kit that is designed to generate amplified sense-strand cDNA ready for fragmentation and labeling using the Affymetrix® GeneChip® WT Terminal Labeling Kit.
Features
- Optimized for use with Affymetrix® GeneChip® Human, Mouse, and Rat 1.0 ST Arrays
- Allows RNA samples as small as 50 ng to be analyzed on Affymetrix® GeneChip® Arrays
- Supports a streamlined workflow that does not require a separate rRNA depletion step
- Is ideal for high-sensitivity expression profiling
Contents
- First-Strand Enzyme Mix (-20°C): 150 µl
- First-Strand Buffer Mix (-20°C): 600 µl
- Second-Strand Enzyme Mix (-20°C): 600 µl
- Second-Strand Buffer Mix (-20°C): 1.45 mL
- IVT Enzyme Mix (-20°C): 850 µl
- IVT Buffer Mix (-20°C): 1.4 mL
- Control RNA 1 mg/ml (-20°C): 10 µl
- 2nd-Cycle Buffer Mix (-20°C): 1.45 mL
- Random Primers (-20°C): 300 µl
- 2nd-Cycle Enzyme Mix (-20°C): 1 mL
- RNase H (-20°C): 300 µl
- Nucleic Acid Binding Buffer Concentrate (room temperature): 12.5 ml
- Nucleic Acid Binding Beads (4°C): 2.5 mL
- Nucleic Acid Wash Solution Concentrate (room temperature): 50 ml
- Elution Solution (4°C or room temperature): 22 ml
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