Invitrogen™ WT Expression Kit, high throughput, 96 Reactions

Invitrogen™ WT Expression Kit, high throughput, 96 Reactions

The WT Expression Kit is an RNA amplification kit that is designed to generate amplified sense-strand cDNA ready for fragmentation and labeling using the Affymetrix® GeneChip® WT Terminal Labeling Kit.

Features

• Optimized for use with Affymetrix® GeneChip® Human, Mouse, and Rat 1.0 ST Arrays
• Allows RNA samples as small as 50 ng to be analyzed on Affymetrix® GeneChip® Arrays
• Supports a streamlined workflow that does not require a separate rRNA depletion step
• Is ideal for high-sensitivity expression profiling

Contents

• First-Strand Enzyme Mix (-20°C): 150 µl
• First-Strand Buffer Mix (-20°C): 600 µl 
• Second-Strand Enzyme Mix (-20°C): 600 µl 
• Second-Strand Buffer Mix (-20°C): 1.45 mL
• IVT Enzyme Mix (-20°C): 850 µl 
• IVT Buffer Mix (-20°C): 1.4 mL 
• Control RNA 1 mg/ml (-20°C): 10 µl 
• 2nd-Cycle Buffer Mix (-20°C): 1.45 mL 
• Random Primers (-20°C): 300 µl 
• 2nd-Cycle Enzyme Mix (-20°C): 1 mL 
• RNase H (-20°C): 300 µl 
• Nucleic Acid Binding Buffer Concentrate (room temperature): 12.5 ml 
• Nucleic Acid Binding Beads (4°C): 2.5 mL
• Nucleic Acid Wash Solution Concentrate (room temperature): 50 ml 
• Elution Solution (4°C or room temperature): 22 ml