Invitrogen™ MessageAmp™ II-Biotin Enhanced aRNA Amplification Kit, 20 Reactions

Invitrogen™ MessageAmp™ II-Biotin Enhanced aRNA Amplification Kit, 20 Reactions

The Ambion® MessageAmp™ II-Biotin Enhanced aRNA Amplification Kit is for the amplification of as little as 50 pg of input RNA for array analysis. The included NTP mix contains biotinylated UTP. The kit includes sufficient reagents for 20 single-round aRNA amplifications.

Expression profiling using gene arrays has become the most widely used method for assessing gene expression on a genome-wide scale. The primary limitation of this technology is the requirement for large amounts of RNA for array hybridization. This hurdle is usually overcome by using T7 RNA Polymerase–driven transcription of cDNA (obtained from total RNA samples) to amplify sufficient amounts of cRNA. This amplification method is the basis of the MessageAmp™ II-Biotin Enhanced aRNA Amplification Kit.

Features

• Detect more genes due to increased percent present calls
• Obtain sufficient amounts of amplified RNA from a single round of amplification with only 50 ng of input RNA
• Biotinylated nucleotide is included in the optimized NTP mix, reducing setup time and maximizing labeling

Contents

• The MessageAmp™ II-Biotin Enhanced aRNA Amplification Kit contains T7 Oligo(dT) Primer, ArrayScript™ RT, RNase Inhibitor,10X First-Strand Buffer, dNTP Mix, Second-Strand Buffer, DNA Polymerase, RNase H, T7 Enzyme Mix, T7 10X Reaction Buffer, Biotin-NTP Mix, and Control RNA (1 mg⁄mL HeLa total RNA) which are all stored at –20°C.
•  The Wash Buffer, cDNA Binding Buffer, aRNA Binding Buffer, 5X Array Fragmentation Buffer, aRNA Filter Cartridges, aRNA Collection Tubes, cDNA Filter Cartridges + Tubes, cDNA Elution Tubes, Nuclease-free Water, and detailed Instruction Manual are stored at room temperature.