Invitrogen™ PARIS™ Kit

Invitrogen™ PARIS™ Kit

The Ambion® PARIS™ system is for the isolation of both RNA and native protein from the same experimental sample. The kit also permits separation of nuclear and cytoplasmic fractions prior to RNA and/or protein isolation. The kit contains sufficient reagents for 50 purifications from1 to 75 mg of tissue or 100 to 107 cells each.

Features

• Fast, simple procedure
• Isolate nuclear and cytoplasmic RNA and protein from cultured cells
• Isolate total RNA and protein from cultured cells or tissues
• No phenol extraction or alcohol precipitation
• Reduces time, cost, and variability between independent experimental samples
  
  

Contents

• Cell Disruption Buffer: 30 mL
• Cell Fractionation Buffer: 25 mL
• Lysis/Binding Solution: 2X 30 mL
• Wash Solution 1: 40 mL
• Wash Solution 2/3 Concentrate: 80 mL
• Lithium Chloride Precipitation Solution: 4 mL
• Filter Cartridges: 50
• Collection Tubes: 100
• Elution Solution: 10 mL