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Invitrogen™ pPICZα A, B, & C Pichia Vectors

Out of stock
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 20 µg
Order number : V19520
$ On demand

Invitrogen™ pPICZα A, B, & C Pichia Vectors

 

pPICα A, B, and C vectors are 3.6 kb vectors used to express and secrete recombinant proteins in Pichia pastoris. Recombinant proteins are expressed as fusions to an N-terminal peptide encoding the Saccharomyces cerevisiae á-factor secretion signal. These vectors allow high-level, methanol inducible expression of the gene of interest in Pichia, and can be used in any Pichia strain including X-33, SMD1168H, and KM71H.


Contents

  • Contains AOX1 promoter for tightly regulated, methanol-induced expression of the gene of interest
  • All three reading frames (A, B, C versions) are provided to facilitate in-frame cloning with the C-terminal peptide
  • α-factor secretion signal for directing secreted expression of the recombinant protein
  • Zeocin resistance gene for selection in both E. coli and Pichia
  • C-terminal peptide containing the c-myc epitope and a polyhistidine (6xHis) tag for detection and purification of a recombinant fusion protein
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Description:
https://www.thermofisher.com/order/catalog/product/V19520?SID=srch-srp-V19520#/V19520?SID=srch-srp-V19520

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