Invitrogen™ pBAD/Myc-His Kit

Invitrogen™ pBAD/Myc-His Kit

The pBAD/Myc-His Kit provides all of the necessary reagents to express your protein in a tightly regulated fashion. The pBAD/Myc-His vector expresses native proteins or fusion proteins with a C-terminal tag

Three vectors are provided (A, B, and C). Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.

Features

• The araBAD promoter for tightly regulated expression
• Translation initiation signals optimized for E. coliexpression
• C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin or detection with an Anti-His(C-term) Antibody
• C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody

Contents

• 20 µg each of pBAD/Myc-His A, B, and C vector in TE Buffer, pH 8.0 (40 µL at 0.5 µg/µl)
• 20 µg each of pBAD/Myc-His/lacZ vector in TE Buffer, pH 8.0 (40 µL at 0.5 µg/µL)
• 1 mL sterile, 20% L-arabinose
• 1 stab LMG194
• 1 stab TOP10