Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli, 25 Reactions

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli, 25 Reactions

The Zero Blunt® TOPO® PCR Cloning Kit for Sequencing provides a highly efficient, 5-minute, one-step cloning strategy for the direct insertion of proofreading-polymerase–amplified, blunt-ended PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4Blunt-TOPO® vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction. These kits include everything necessary to clone and select recombinant vectors containing your PCR fragment of choice.

Features

• We have removed much of the multiple cloning site from the pCR™4Blunt-TOPO® vector to shorten the distance between sequencing primer sites and the insert site to as little as 33 bp. This means sequencing reactions give less vector sequence and more insert sequence.
• The pCR™4Blunt-TOPO® vector contains both ampicillin and kanamycin resistance markers and a LacZα-ccdB gene fusion for positive selection
• There is no need for PCR primers containing specific sequences, post-PCR procedures, vector preparation, or other time-intensive DNA manipulation steps.
• Up to 95% of clones carrying the desired insert
• Fast, simple-to-use, and efficient, TOPO® cloning has been applied to many different vectors for a wide array of applications.
• The Zero Blunt® TOPO® PCR Cloning Kit for Sequencing can be purchased with a variety of competent cells that deliver different advantages depending on what your needs

Contents

• Linearized and topoisomerase I-activated pCR™4-Blunt TOPO™ Vector
• Salt Solution
• dNTPs
• Control Template and Primers
• M13 Forward and Reverse Primers
• One Shot™ Chemically Competent TOP10 E. coli
• S.O.C. Medium
• Supercoiled control plasmid.