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Invitrogen™ pcDNA™3.1 (-) Mammalian Expression Vector

Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 20 µg
Order number : V79520
($) On demand

Invitrogen™ pcDNA™3.1 (-) Mammalian Expression Vector

 

This pcDNA™3.1(+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site.

The pcDNA™3.1 Expression Vector Family

Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections.

 

Features

  • Cytomegalovirus (CMV) enhancer-promoter for high-level expression
  • Large multiple cloning site in either forward (+) or reverse (-) orientations
  • Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
  • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e., COS-1 and COS-7)
  • Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

 

Contents

  • pcDNA™3.1(–): 40 μL
  • Control Plasmid pcDNA™3.1/CAT: 40 μL
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