Invitrogen™ pcDNA™3.1 (-) Mammalian Expression Vector
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
20 µg
Order number :
V79520
Invitrogen™ pcDNA™3.1 (-) Mammalian Expression Vector
This pcDNA™3.1(+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site.
The pcDNA™3.1 Expression Vector Family
Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections.
Features
- Cytomegalovirus (CMV) enhancer-promoter for high-level expression
- Large multiple cloning site in either forward (+) or reverse (-) orientations
- Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
- SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e., COS-1 and COS-7)
- Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
Contents
- pcDNA™3.1(–): 40 μL
- Control Plasmid pcDNA™3.1/CAT: 40 μL