Loader

Invitrogen™ pTracer™-SV40 Mammalian Expression Kit

Out of stock
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 1 Kit
Order number : V87001
($) On demand

Invitrogen™ pTracer™-SV40 Mammalian Expression Kit

 

Three pTracer™ mammalian expression vectors are available that express GFP fused to the selectable marker Zeocin™ . Each vector uses a different set of promoters to express the gene of interest and the Cycle 3 GFP-Zeocin™ fusion.

The pTracer™-SV40 vector offers:

  • SV40 promoter for high-level constitutive expression of your gene of interest
  • CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion
  • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the SV40 large T antigen

The pTracer™-CMV2 vector offers:

  • CMV promoter for high-level constitutive expression of your gene of interest
  • EF-1α promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion
  • Ampicillin resistance gene for selection in E. coli

The pTracer™-EF vectors offer:

  • EF-1α promoter for high-level constitutive expression of your gene of interest
  • CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion
  • C-terminal V5 epitope tag for simple detection of recombinant fusion proteins with an Anti-V5 Antibody
  • C-terminal 6xHis tag for rapid purification on nickel-chelating resin and simple detection with an Anti-His(C-term) Antibody
  • Multiple cloning site supplied in three reading frames to simplify cloning in frame with the C-terminal tag

 

Features

  • Cycle 3 GFP-Zeocin™ fusion for selection in mammalian cell lines
  • BGH polyA signal and transcription termination sequences to enhance mRNA stability

 

Contents

  • pTracer™-SV40: 40 µL
  • Zeocin™ antibiotic:  1 g (100 mg/mL, 8 × 1.25 mL)
More  
Description:

Images:

No attachment


Files:

No attachment

Video:

You May Also Like