Invitrogen™ pTracer™-SV40 Mammalian Expression Kit
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
1 Kit
Order number :
V87001
Invitrogen™ pTracer™-SV40 Mammalian Expression Kit
Three pTracer™ mammalian expression vectors are available that express GFP fused to the selectable marker Zeocin™ . Each vector uses a different set of promoters to express the gene of interest and the Cycle 3 GFP-Zeocin™ fusion.
The pTracer™-SV40 vector offers:
- SV40 promoter for high-level constitutive expression of your gene of interest
- CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion
- SV40 origin for episomal replication and simple vector rescue in cell lines expressing the SV40 large T antigen
The pTracer™-CMV2 vector offers:
- CMV promoter for high-level constitutive expression of your gene of interest
- EF-1α promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion
- Ampicillin resistance gene for selection in E. coli
The pTracer™-EF vectors offer:
- EF-1α promoter for high-level constitutive expression of your gene of interest
- CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion
- C-terminal V5 epitope tag for simple detection of recombinant fusion proteins with an Anti-V5 Antibody
- C-terminal 6xHis tag for rapid purification on nickel-chelating resin and simple detection with an Anti-His(C-term) Antibody
- Multiple cloning site supplied in three reading frames to simplify cloning in frame with the C-terminal tag
Features
- Cycle 3 GFP-Zeocin™ fusion for selection in mammalian cell lines
- BGH polyA signal and transcription termination sequences to enhance mRNA stability
Contents
- pTracer™-SV40: 40 µL
- Zeocin™ antibiotic: 1 g (100 mg/mL, 8 × 1.25 mL)
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