Invitrogen™ Silencer™ siRNA Labeling Kit with FAM dye

Invitrogen™ Silencer™ siRNA Labeling Kit with FAM dye

For the labeling of siRNA with Cy™3. Includes sufficient reagents for labeling 65 µg of siRNA. Ambion® Labeled siRNA can be used to analyze siRNA subcellular localization, stability, and transfection efficiency. In addition, fluorescently labeled siRNA is particularly well-suited for use in double-label experiments (with a labeled antibody) to track cells that receive siRNA during transfection and to correlate transfection with down-regulation of the target protein. Research shows that labeling of siRNAs does not affect their biological function.

Features

• Includes Label or Dye: Yes
• Labeling Method: Direct Labeling
• Product Line: Silencer™, Ambion™
• Final Product Type: siRNA (Labeled)
• Label or Dye: FITC (Fluorescein)
• Labeling Target: siRNA
• Detection Method: Fluorescent

Contents

• Nuclease-free Water: 1.75 mL
• 10X Labeling Buffer: 500 μL
• Reconstitution Solution: 100 μL
• 5M NaCl: 500 μL
• 5X siRNA Annealing Buffer: 500 μL
• GAPDH siRNA 20 μM solution: ~10 μg total: 40 μL
• Cy®3 Labeling Reagent: 40 μg      

Related Accessories

• Also available is the Silencer® Cy™3 siRNA Labeling Kit: AM1632