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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with pCR™4-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit

Out of stock
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 25 Reactions
Order number : K457502
($) On demand

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with pCR™4-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit


The TOPO® TA Cloning® Kits for Sequencing provide a highly efficient, 5 minute, one-step cloning strategy ('TOPO® Cloning') for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4-TOPO® TA vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction. These kits include everything necessary to clone and select recombinant vectors containing your PCR fragment of choice.


Features

  • Get More Sequence—Allows for more insert sequence and less vector seuquence when using standard sequencing primers
  • Fast and Easy—Go from PCR-to-clones in just 3 steps and in as little as 5 minutes hands-on time
  • Efficient—Achieve up to 95% clones with correct insert
  • Proven—Reliable performance for over a decade with over 4000 citations

 

Contents


Box 1:

  • Topoisomerase I-activated pCR™4-TOPO™ vector
  • PCR buffer
  • Salt solution
  • dNTPs
  • Control template
  • T3, T7, M13 forward, and M13 reverse primers
  • Control PCR primers
  • Sterile water

Box 2:

  • One Shot™ Chemically Competent or Electrocomp™ E. coli
  • S.O.C. medium
  • Supercoiled pUC19 control plasmid

Box 3:

  • PureLink Quick Plasmid Miniprep Kit
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