Thermo Scientific™ Active Ras Pull-Down and Detection Kit

Thermo Scientific™ Active Ras Pull-Down and Detection Kit

The Thermo Scientific Active Ras Pull-Down and Detection Kit enables selective enrichment and detection of GTP-bound Ras GTPase through a specific protein interaction with the Raf1 protein-binding domain.

The Active Ras Pull-Down and Detection Kit includes purified GST-Raf1 Ras-binding domain (RBD), glutathione agarose resin, positive and negative controls (GTPγS and GDP, respectively), lysis/binding/washing buffer, anti-Ras antibody, SDS sample buffer, spin columns and collection tubes. The kit was validated using lysates from NIH 3T3 cells, a cell line known to have robust Ras activity.

Features

• Highly sensitive and accurate—optimized reagents, specific anti-Ras antibody and Western blot procedure ensure accurate controls and semi-quantitative results
• Validated—functionally tested for Ras detection to ensure quality and performance
• Compatible—effective for a variety of cell types from mouse, rat and human sources

Contents

• GST-Raf1-RBD Fusion Protein, 1 vial 
• Anti-Ras Antibody, 5 units, 250 µL 
• Glutathione Agarose Resin, 3 mL 
• GDP (100X), 50 µL 
• GTPyS (100X), 50 µL 
• SDS Sample Buffer (2X), 1.5 mL 
• Lysis/Binding/Wash Buffer (1X), 100 mL 
• Spin Cups, 30 cups 
• Collection Tubes, 90 tubes